Purpose: In this lab, you are going to assess the value of that hand-washing advice and the methods that bring the most benefit from hand washing. Examine the results of the handwashing experiment and understand the various interpretations. Wet your hands with clean, running water (warm or cold) and apply soap. . Compare the effectiveness of hand washing with water, with soap, and with soap and surgical scrubbing. Wash thoroughly with your washing agent for two (2) minutes. Take two sterile … Before birth, the skin of a human being is sterile.  During the birthing process and subsequently, the human skin is colonized by microorganisms.  The set of microorganisms that permanently colonize the human skin and do not cause harm to a healthy individual is called the residential flora.  The set of microorganisms that are present only temporarily on the skin is called the transient flora.  The residential flora tends to be located in the deeper layers of the epidermis and in the hair follicles.  The transient flora is located superficially.  The residential flora is more difficult to remove from the skin than the transient flora.  The residential flora exists in a symbiotic relationship with the host.  By competing for resources with pathogenic microorganisms, the resident flora can provide benefit to the host (Cappuccino 7). ... we took a culture from a fingertip of a student’s unwashed hands and a culture after a thorough hand washing. I (Lauren) joined the team just before the … Continue rubbing your hands for at least 20 seconds. The exercise compares the effectiveness of two hand washing techniques: using water only, and using soap and water. is more effective in reducing bacteria when used with. The students investigate the scientific method by a prompt in the form of a letter asking them to design a hand washing experiment. How does hand washing affect residential versus transient flora? The agar plates were visually examined after the period of incubation and the observations appear in Table 1.1.  The growth for the “Water” plates starts heavy and progressively diminishes to slight growth.  The growth for the “Soap” plates starts at heavy and also diminishes to slight growth but shows lag in the rate of decrease at the beginning. Hot Water, Hand washing is an important method of prevention against possible, infections caused by pathogens. Student 1 uses aseptic technique to soak a sterile cotton swab in sterile saline solution in a test tube.  Student 1 then proceeds to rub the moistened cotton swab on the pad of the left hand thumb of Student 2 and then inoculates the L1 section of the “Soap” plate using a zigzag pattern from the edge of the dish towards the dividing middle line. To clean our hands, the Centers for Disease Control and Prevention recommends that we: wet hands with clean water. Required fields are marked *. Web. According to research literature, hand washing with water only is ineffective.  Using soap for extended periods of time is a better alternative to using only water.  However, this allows some transient flora to cling deeply to hair and to remain protected by oil and dead skin cells.  Soap with surgical scrubbing is a good way to remove transient flora and some residential flora. In addition to hand bacteria, hands were inoculated with table surface bacteria. Correct hand-washing is the single most effective technique that prevents the transmission of micro-organisms either directly or indirectly to others. Food can additionally become contaminated through inadequate sanitation, cross-contamination and the improper application … Lab Exercise 1-1 (Proper Hand-Washing Technique).....6 Lab Exercise 1-2 (Bacteria are Ubiquitous) ... Report any injury to your instructor. It is routine practice to wash the hands prior to and after examining a … We printed our fingers in petri dishes before hand washing, after poor hand washing, and after proper hand washing, then incubated them overnight to allow the bacteria to grow. 5. The benefits of liquid soap include: Hygiene – it … Table 1.1 Macroscopic observations of microorganism growth. Transient flora are the microorganisms you pick up from what you have been handling. Don’t forget the backs of your hands, your wrists, between your fingers … Residential flora is difficult to remove with simple hand washing.  Transient flora is easier to remove.  Surgical scrubbing is the best way to remove transient flora and reduce the presence of residential flora. RePort Laboratory Fall2008 MicrobiologY handwashins/fomites the data from the interpreting and report summarizing Write a into the It should be divided your wellwritten. This laboratory exercise illustrates the critical need for proper hand washing techniques as a means of reducing the incidence of healthcare facility acquired infections.  The exercise compares the effectiveness of two hand washing techniques: using water only, and using soap and water.  The student is exposed to the fact that the hands harbor microorganisms that could result in transmitting disease.  In addition, the microorganisms resident in the hands could result in contamination of laboratory experiments if aseptic techniques, such as wearing gloves, are not strictly followed. apply soap and lather/scrub every nook and cranny of your hands for 20-30 seconds (about the time to sing "Happy Birthday" twice). The participants in the experiment are Student 1 and Student 2. Liquid soap is better than bar soap, especially at work. There are 2 categories of microorganisms, or flora, normally found on the hands. . Microbiology 1. The experiment should be modified to have a better starting condition for the hand contamination.  Both hands should be used to contact the same surfaces where microorganisms are known to be present. The procedure consists of the following steps.  Using the glass marking pencil, Student 1 marks the bottom of two agar plates with the label “Water” and the other two plates with the label “Soap”.  The Student 1 then marks the bottom of each “Water” and “Soap” plate with a dividing middle line.  Each half of a “Water” plate is labeled R1 and R2 respectively.  Each half of the other “Water” plate is labeled R3 and R4 respectively.  Each half of a “Soap” plate is labeled L1 and L2 respectively.  Each half of the other “Soap” plate is labeled L3 and L4 respectively. Thoroughly washing hands is the single most important thing students can do to keep from getting sick, or to keep from infecting others. The materials for this laboratory experiment include: 4 nutrient agar plates; liquid antibacterial soap; 8 sterile cotton swabs, 1 test tube of sterile saline; Bunsen burner, glass marking pencil; stopwatch. Boston: Pearson, 2014. Materials Distilled water Test tube 6 Unopened packages of 1 sterile cotton swab 2 sterile … . Start studying Microbiology Lab: Effectiveness of Hand Washing. So here is a … Apply one dose of liquid soap and lather (wash) well for 15–20 seconds (or longer if the dirt is ingrained). as microbiology cannot be achieved effectively without enhancing the theory with “hands on” experience in the laboratory. IMPORTANCE OF HAND HYGIENE 3 Materials and Methods Several materials were used in order to conduct this experiment. Be sure to scrub the backs of your hands, between your fingers, and under your nails. Rub hands together rapidly across all surfaces of your hands and wrists to help remove dirt and germs. Hand Wash Lab Introduction: You are aware that you have been taught to wash your hands often, especially before eating and after; well, you know. Two hand-washing methods, using hot and cold water with soap, were tested through colonial growth observation to determine how successful they were in the removal of bacteria. Print. Hand Washing Video by CDC Student 1 uses aseptic technique to soak a sterile cotton swab in sterile saline solution in a test tube.  The aseptic technique consists of removing the cap of the test tube, flaming the neck of the test tube using the Bunsen burner, dipping the cotton swab in saline, and putting the cap back on the test tube.  Student 1 then proceeds to rub the moistened cotton swab on the pad of the right hand thumb of Student 2 and then inoculates the R1 section of the “Water” plate using a zigzag pattern from the edge of the dish towards the dividing middle line. Share; Like; Download ... MBBS IMS MSU. Microbiology Lab Practices and Safety Rules 1. good laboratory practices and technique, (2) safety equipment, and (3) facility design. This experiment asks them to test antimicrobial chemicals for use in soaps. The term “hand hygiene” represents a new term in the healthcare vocabulary, replacing the more narrow term of “handwashing”. In addition to hand bacteria, hands were inoculated with, Results of the hand-washing test showed that a thorough, cleansing of the hands with soap and water reduces bacterial numbers and therefore. “Infection control in dental settings,” cdc.gov.  Centers for disease control and prevention. Picks: Celebrating a year of incredible Prezi videos ; Dec. 1, 2020 results of the.! ( lab report is known as the elaborate explanation of a particular selected that! Only, and ( 3 ) facility design the left hand thumb do... This preview shows page 1 - 2 out of 4 pages getting sick, to! 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